Piotr E. Marszalek

Research Themes

Biomechanics & Biomaterials, Soft Matter & Nanoscale Materials

Research Interests

Investigating relationships between structural and mechanical properties of biopolymers (polysaccharides, DNA, proteins), at a single molecule level.

Bio

My research focuses on investigating relationships between structural and mechanical properties of biopolymers (polysaccharides, DNA, proteins), which I study at a single molecule level. My main approaches are experimental scanning probe microscopy techniques and computational methods involving Molecular Dynamics simulations and ab initio quantum mechanical calculations. The ultimate goal of this research is to understand the above-mentioned relationships at an atomic level and to apply the knowledge gained towards elucidating basic phenomena such as: molecular recognition that mediates interactions between proteins and sugars, mechanotransduction that underlies mechanical sensing and hearing in all organisms, and protein folding that is fundamental to all biology. Our DNA research is aimed at exploiting atomic force microscopy techniques to develop new ultra-sensitive assays for detecting and examining DNA damage, the process underlying carcinogenesis, and to increase our mechanistic understanding of DNA damage and repair processes. This research, in addition to its basic science aspects will lay a foundation for the future use of AFM technologies in the nanoscale DNA diagnostics with a potential to directly benefit human health.

Education

• M.S. University of Warsaw (Poland), 1985
• Ph.D. Electrotechnical Institute (Poland), 1991

Positions

• Professor in the Thomas Lord Department of Mechanical Engineering and Materials Science

Courses Taught

• ME 513: Nanobiomechanics
• ME 391: Undergraduate Projects in Mechanical Engineering
• ME 331L: Thermodynamics
• EGR 393: Research Projects in Engineering
• BME 494: Projects in Biomedical Engineering (GE)
• BME 493: Projects in Biomedical Engineering (GE)
• BME 394: Projects in Biomedical Engineering (GE)

Publications

• Apostolidou D, Zhang P, Pandya D, Bock K, Liu Q, Yang W, et al. Tandem repeats of highly bioluminescent NanoLuc are refolded noncanonically by the Hsp70 machinery. Protein science : a publication of the Protein Society. 2024 Feb;33(2):e4895.
• Kim S, Cathey MVJ, Bounds BC, Scholl Z, Marszalek PE, Kim M. Ligand-Mediated Mechanical Enhancement in Protein Complexes at Nano- and Macro-Scale. ACS applied materials & interfaces. 2024 Jan;16(1):272–80.
• Antosiewicz JM, Gilbert R, Marszalek PE. Special Issue: 18th Congress of the Polish Biophysical Society. European biophysics journal : EBJ. 2023 Oct;52(6–7):483–6.
• Marszalek PE. Capturing intrinsic nanomechanics of allostery. Biophysical journal. 2022 Dec;121(23):4415–6.
• Apostolidou D, Zhang P, Yang W, Marszalek PE. Mechanical Unfolding and Refolding of NanoLuc via Single-Molecule Force Spectroscopy and Computer Simulations. Biomacromolecules. 2022 Dec;23(12):5164–78.
• Li Q, Apostolidou D, Marszalek PE. Reconstruction of mechanical unfolding and refolding pathways of proteins with atomic force spectroscopy and computer simulations. Methods (San Diego, Calif). 2022 Jan;197:39–53.
• Zhang P, Wang D, Yang W, Marszalek PE. Piecewise All-Atom SMD Simulations Reveal Key Secondary Structures in Luciferase Unfolding Pathway. Biophysical journal. 2020 Dec;119(11):2251–61.
• Ding Y, Apostolidou D, Marszalek P. Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc. International journal of molecular sciences. 2020 Dec;22(1):E55.
• Marszalek PE, Oberhauser AF. Meeting report - NSF-sponsored workshop 'Progress and Prospects of Single-Molecule Force Spectroscopy in Biological and Chemical Sciences'. Journal of cell science. 2020 Aug;133(16):jcs251421.
• Wang D, Marszalek PE. Exploiting a Mechanical Perturbation of a Titin Domain to Identify How Force Field Parameterization Affects Protein Refolding Pathways. Journal of chemical theory and computation. 2020 May;16(5):3240–52.
• Endow SA, Marszalek PE. An estimate to the first approximation of microtubule rupture force. Eur Biophys J. 2019 Sep;48(6):569–77.
• Scholl ZN, Li Q, Josephs E, Apostolidou D, Marszalek PE. Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope. Journal of visualized experiments : JoVE. 2019 Feb;(144).
• Scholl ZN, Josephs EA, Marszalek PE. Correction to "Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy". Biomacromolecules. 2019 Jan;20(1):567.
• Josephs EA, Marszalek PE. Endonuclease-independent DNA mismatch repair processes on the lagging strand. DNA repair. 2018 Aug;68:41–9.
• Li Q, Scholl ZN, Marszalek PE. Unraveling the Mechanical Unfolding Pathways of a Multidomain Protein: Phosphoglycerate Kinase. Biophysical journal. 2018 Jul;115(1):46–58.
• Plata CA, Scholl ZN, Marszalek PE, Prados A. Relevance of the Speed and Direction of Pulling in Simple Modular Proteins. Journal of chemical theory and computation. 2018 Jun;14(6):2910–8.
• Gimsa J, Wysotzki P, Perutkova Š, Weihe T, Elter P, Marszałek P, et al. Spermidine-Induced Attraction of Like-Charged Surfaces Is Correlated with the pH-Dependent Spermidine Charge: Force Spectroscopy Characterization. Langmuir : the ACS journal of surfaces and colloids. 2018 Feb;34(8):2725–33.
• Scholl ZN, Marszalek PE. AFM-Based Single-Molecule Force Spectroscopy of Proteins. Methods in molecular biology (Clifton, NJ). 2018 Jan;1814:35–47.
• Marszalek PE. Warhammers for Peaceful Times. Biophysical journal. 2018 Jan;114(1):1–2.
• Scholl ZN, Yang W, Marszalek PE. Competing Pathways and Multiple Folding Nuclei in a Large Multidomain Protein, Luciferase. Biophysical journal. 2017 May;112(9):1829–40.
• Josephs EA, Marszalek PE. A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches. Nucleic acids research. 2017 May;45(8):e63.
• Josephs EA, Marszalek PE, Scholl ZN. Atomic force microscope force spectroscopy. In: An Introduction to Single Molecule Biophysics. 2017. p. 79–114.
• Gonzalez MA, Simon JR, Ghoorchian A, Scholl Z, Lin S, Rubinstein M, et al. Strong, Tough, Stretchable, and Self-Adhesive Hydrogels from Intrinsically Unstructured Proteins. Advanced Materials. 2017;
• Benedetti F, Gazizova Y, Kulik AJ, Marszalek PE, Klinov DV, Dietler G, et al. Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment? Biophysical journal. 2016 Sep;111(6):1163–72.
• Scholl ZN, Li Q, Yang W, Marszalek PE. Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a βγ-Crystallin Protein. The Journal of biological chemistry. 2016 Aug;291(35):18263–75.
• Scholl ZN, Josephs EA, Marszalek PE. Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy. Biomacromolecules. 2016 Jul;17(7):2502–5.
• Kelner JM, Ziolkowski C, Marszalek P. Influence of the frequency stability on the emitter position in SDF method. In: 2016 International Conference on Military Communications and Information Systems, ICMCIS 2016. 2016.
• Josephs EA, Kocak DD, Fitzgibbon CJ, McMenemy J, Gersbach CA, Marszalek PE. Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage. Nucleic acids research. 2016 Mar;44(5):2474.
• Josephs EA, Zheng T, Marszalek PE. Atomic force microscopy captures the initiation of methyl-directed DNA mismatch repair. DNA repair. 2015 Nov;35:71–84.
• Josephs EA, Kocak DD, Fitzgibbon CJ, McMenemy J, Gersbach CA, Marszalek PE. Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage. Nucleic acids research. 2015 Oct;43(18):8924–41.
• Scholl ZN, Yang W, Marszalek PE. Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. ACS nano. 2015 Feb;9(2):1189–97.
• Scholl ZN, Yang W, Marszalek PE. Erratum: Chaperones rescue luciferase folding by separating its domains (Journal of Biological Chemistry (2014) 289 (28607-28618) DOI: 10.1074/jbc.A114.582049). Journal of Biological Chemistry. 2015 Jan 9;290(2):883.
• Jagusiak A, Konieczny L, Krol M, Marszalek P, Piekarska B, Piwowar P, et al. Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool. Mini reviews in medicinal chemistry. 2015 Jan;14(13):1104–13.
• Li Q, Scholl ZN, Marszalek PE. Capturing the mechanical unfolding pathway of a large protein with coiled-coil probes. Angewandte Chemie (International ed in English). 2014 Dec;53(49):13429–33.
• Malon K, Skokowski P, Marszalek P, Kelner JM, Lopatka J. Cognitive manager for hierarchical cluster networks based on multi-stage machine method. In: Proceedings - IEEE Military Communications Conference MILCOM. 2014. p. 428–33.
• Scholl ZN, Yang W, Marszalek PE. Chaperones rescue luciferase folding by separating its domains. The Journal of biological chemistry. 2014 Oct;289(41):28607–18.
• Scholl ZN, Marszalek PE. Unraveling the mysteries of chaperone interactions of the myosin head. Biophysical journal. 2014 Aug;107(3):541–2.
• Scholl ZN, Li Q, Marszalek PE. Single molecule mechanical manipulation for studying biological properties of proteins, DNA, and sugars. Wiley interdisciplinary reviews Nanomedicine and nanobiotechnology. 2014 May;6(3):211–29.
• Scholl ZN, Marszalek PE. Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions. Ultramicroscopy. 2014 Jan;136:7–14.
• Scholl ZN, Rabbi M, Lee D, Manson L, S-Gracz H, Marszalek PE. Origin of overstretching transitions in single-stranded nucleic acids. Physical review letters. 2013 Nov 1;111(18):188302.
• Scholl ZN, Rabbi M, Lee D, Manson L, S-Gracz H, Marszalek PE. Origin of overstretching transitions in single-stranded nucleic acids. Physical review letters. 2013 Nov;111(18):188302.
• Dendzik M, Kulik A, Benedetti F, Marszalek PE, Dietler G. A single-molecule stretching method for lateral and normal AFM lever calibration. Nanotechnology. 2013 Sep;24(36):365703.
• Settanni G, Serquera D, Marszalek PE, Paci E, Itzhaki LS. Effects of ligand binding on the mechanical properties of ankyrin repeat protein gankyrin. PLoS computational biology. 2013 Jan;9(1):e1002864.
• Loksztejn A, Scholl Z, Marszalek PE. Atomic force microscopy captures folded ribosome bound nascent chains. Chemical communications (Cambridge, England). 2012 Dec;48(96):11727–9.
• Lee W, Strümpfer J, Bennett V, Schulten K, Marszalek PE. Erratum: Mutation of conserved histidines alters tertiary structure and nanomechanics of consensus ankyrin repeats (Journal of Biological Chemistry (2012) 287, (19115-19121) DOI: 10.1074/jbc.A112.365569). Journal of Biological Chemistry. 2012 Oct 12;287(42):35539.
• Lee W, Strümpfer J, Bennett V, Schulten K, Marszalek PE. Mutation of conserved histidines alters tertiary structure and nanomechanics of consensus ankyrin repeats. J Biol Chem. 2012 Jun 1;287(23):19115–21.
• Marszalek PE, Dufrêne YF. Stretching single polysaccharides and proteins using atomic force microscopy. Chemical Society reviews. 2012 May;41(9):3523–34.
• Callahan DJ, Liu W, Li X, Dreher MR, Hassouneh W, Kim M, et al. Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution. Nano letters. 2012 Apr;12(4):2165–70.
• Lee W, Zeng X, Rotolo K, Yang M, Schofield CJ, Bennett V, et al. Mechanical anisotropy of ankyrin repeats. Biophys J. 2012 Mar 7;102(5):1118–26.
• Kim M, Wang C-C, Benedetti F, Marszalek PE. A nanoscale force probe for gauging intermolecular interactions. Angewandte Chemie (International ed in English). 2012 Feb;51(8):1903–6.
• Kim M, Wang C-C, Benedetti F, Rabbi M, Bennett V, Marszalek PE. Nanomechanics of streptavidin hubs for molecular materials. Adv Mater. 2011 Dec 15;23(47):5684–8.
• Jiang Y, Marszalek PE. Atomic force microscopy captures MutS tetramers initiating DNA mismatch repair. The EMBO journal. 2011 Jun;30(14):2881–93.
• Wang CC, Tsong TY, Hsu YH, Marszalek PE. Erratum: Inhibitor binding increases the mechanical stability of staphylococcal nuclease (Biophysical Journal (2011) 104 (1094-1099)). Biophysical Journal. 2011 Mar 16;100(6):1596.
• Lee W, Zeng X, Zhou HX, Bennett V, Yang W, Marszalek PE. Full reconstruction of a vectorial protein folding pathway by atomic force microscopy and molecular dynamics simulations. Journal of Biological Chemistry. 2011 Mar 11;286(10):8708.
• Wang C-C, Tsong T-Y, Hsu Y-H, Marszalek PE. Inhibitor binding increases the mechanical stability of staphylococcal nuclease. Biophysical journal. 2011 Feb;100(4):1094–9.
• Lee W, Marszalek PE, Zeng X, Yang W. Mechanics of polysaccharides. In: Molecular Manipulation with Atomic Force Microscopy. 2011. p. 83–128.
• Lee W, Zeng X, Zhou H-X, Bennett V, Yang W, Marszalek PE. Full reconstruction of a vectorial protein folding pathway by atomic force microscopy and molecular dynamics simulations. J Biol Chem. 2010 Dec 3;285(49):38167–72.
• Marszalek PE. DNA sequencing: detecting methylation with force. Nature nanotechnology. 2010 Nov;5(11):765–6.
• Jiang Y, Rabbi M, Mieczkowski PA, Marszalek PE. Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis. The journal of physical chemistry B. 2010 Sep;114(37):12162–5.
• Kim M, Abdi K, Lee G, Rabbi M, Lee W, Yang M, et al. Fast and forceful refolding of stretched alpha-helical solenoid proteins. Biophys J. 2010 Jun 16;98(12):3086–92.
• Serquera D, Lee W, Settanni G, Marszalek PE, Paci E, Itzhaki LS. Mechanical unfolding of an ankyrin repeat protein. Biophysical journal. 2010 Apr;98(7):1294–301.
• Zeng X, Hu H, Zhou H-X, Marszalek PE, Yang W. Equilibrium sampling for biomolecules under mechanical tension. Biophysical journal. 2010 Feb;98(4):733–40.
• Glowacki M, Budohoski K, Marszalek P, Walecki J, Czernicki Z. A non-invasive assessment of intracranial volume reserve by measuring cerebrospinal fluid volume with the aid of CT imaging. Acta neurochirurgica Supplement. 2010 Jan;106:199–202.
• Lee G, Muramoto GG, Chute JP, Marszalek PE. Nanomechanical fingerprints of gamma radiation damage to DNA. Journal of nanoscience and nanotechnology. 2009 Dec;9(12):7359–63.
• Rivera M, Lee W, Marszalek PE, Cole DG, Clark RL. Aligning molecular attachment sites in single molecule force spectroscopy measurements. 2008 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC 2008. 2009 Nov 23;4:663–7.
• Ke C, Loksztejn A, Jiang Y, Kim M, Humeniuk M, Rabbi M, et al. Detecting solvent-driven transitions of poly(A) to double-stranded conformations by atomic force microscopy. Biophysical journal. 2009 Apr;96(7):2918–25.
• Janke M, Rudzevich Y, Molokanova O, Metzroth T, Mey I, Diezemann G, et al. Mechanically interlocked calix[4]arene dimers display reversible bond breakage under force. Nature nanotechnology. 2009 Apr;4(4):225–9.
• Jiang Y, Rabbi M, Kim M, Ke C, Lee W, Clark RL, et al. UVA generates pyrimidine dimers in DNA directly. Biophysical journal. 2009 Feb;96(3):1151–8.
• Rivera M, Lee W, Marszalek PE, Cole DG, Clark RL. Aligning molecular attachment sites in single molecule force spectroscopy measurements. Proceedings of the ASME Design Engineering Technical Conference. 2008 Dec 1;4:663–7.
• Rivera M, Lee W, Ke C, Marszalek PE, Cole DG, Clark RL. Minimizing pulling geometry errors in atomic force microscope single molecule force spectroscopy. Biophysical journal. 2008 Oct;95(8):3991–8.
• Rivera M, Ke C, Marszalek PE, Cole DG, Clark RL. A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements. 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007. 2008 Jun 13;3 PART B:801–7.
• Ke C, Jiang Y, Mieczkowski PA, Muramoto GG, Chute JP, Marszalek PE. Nanoscale detection of ionizing radiation damage to DNA by atomic force microscopy. Small (Weinheim an der Bergstrasse, Germany). 2008 Feb;4(2):288–94.
• Jiang Y, Ke C, Mieczkowski PA, Marszalek PE. Detecting ultraviolet damage in single DNA molecules by atomic force microscopy. Biophysical journal. 2007 Sep;93(5):1758–67.
• Ke C, Humeniuk M, S-Gracz H, Marszalek PE. Direct measurements of base stacking interactions in DNA by single-molecule atomic-force spectroscopy. Physical review letters. 2007 Jul;99(1):018302.
• Ke C, Jiang Y, Rivera M, Clark RL, Marszalek PE. Pulling geometry-induced errors in single molecule force spectroscopy measurements. Biophysical journal. 2007 May;92(9):L76–8.
• Lee G, Rabbi M, Clark RL, Marszalek PE. Nanomechanical fingerprints of UV damage to DNA. Small (Weinheim an der Bergstrasse, Germany). 2007 May;3(5):809–13.
• Snyder PW, Lee G, Marszalek PE, Clark RL, Toone EJ. A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities. Proceedings of the National Academy of Sciences of the United States of America. 2007 Feb;104(8):2579–84.
• Lee GR, Abdi K, Bennett V, Marszalek PE. The mechanical behavior of beta-catenin captured by an atomic force microscopy. Biophysical Journal. 2007;659A-659A.
• Ke C, Jiang Y, Muramoto GG, Chute JP, Marszalek PE. Direct detection of gamma radiation-induced DNA damage at a single molecule level by AFM. BIOPHYSICAL JOURNAL. 2007 Jan 1;165A-165A.
• Lee G, Lee W, Marszalek PE. Nanomechanics of alginate molecules by single molecule force spectroscopy. BIOPHYSICAL JOURNAL. 2007 Jan 1;657A-657A.
• Lu Z, Hu H, Yang W, Marszalek PE. Simulating force-induced conformational transitions in polysaccharides with the SMD replica exchange method. Biophysical journal. 2006 Sep;91(6):L57–9.
• Zhang Q, Lu Z, Hu H, Yang W, Marszalek PE. Direct detection of the formation of V-amylose helix by single molecule force spectroscopy. Journal of the American Chemical Society. 2006 Jul;128(29):9387–93.
• Zhang Q, Marszalek PE. Identification of sugar isomers by single-molecule force spectroscopy. Journal of the American Chemical Society. 2006 May;128(17):5596–7.
• Lee G, Abdi K, Jiang Y, Michaely P, Bennett V, Marszalek PE. Nanospring behaviour of ankyrin repeats. Nature. 2006 Mar 9;440(7081):246–9.
• Zhang QM, Marszalek PE. Solvent effects on the elasticity of polysaccharide molecules in disordered and ordered states by single-molecule force spectroscopy. Polymer. 2006;47(7):2526–32.
• Cieplak M, Marszalek PE. Mechanical unfolding of ubiquitin molecules. The Journal of chemical physics. 2005 Nov 15;123(19):194903.
• Dzwolak W, Marszalek PE. Zipper-like properties of [poly(L-lysine)+ poly(L-glutamic acid)]beta-pleated molecular self-assembly. Chemical communications (Cambridge, England). 2005 Nov;(44):5557–9.
• Cieplak M, Marszalek PE. Mechanical unfolding of ubiquitin molecules. The Journal of chemical physics. 2005 Nov;123(19):194903.
• Zhang Q, Jaroniec J, Lee G, Marszalek PE. Direct detection of inter-residue hydrogen bonds in polysaccharides by single-molecule force spectroscopy. Angewandte Chemie (International ed in English). 2005 Apr;44(18):2723–7.
• Zhang QM, Jaroniec J, Lee GR, Marszalek PE. Direct detection of inter-residue hydrogen bonds in polysaccharides by single molecule force spectroscopy. BIOPHYSICAL JOURNAL. 2005 Jan 1;88(1):170A-170A.
• Zhang QM, Jaronic J, Lee G, Marszalek P. Detection of inter-residue hydrogen bonds in polysaccharides by AFM. Abstracts of Papers of the American Chemical Society. 2005;229:U788–U788.
• Jaroniec J, Marszalek PE. A quantum mechanical study of forced conformational transitions in sugar rings. BIOPHYSICAL JOURNAL. 2005 Jan 1;88(1):169A-169A.
• Jarus-Dziedzic K, Jurkiewicz J, Czernicki Z, Fersten E, Głowacki M, Marszałek P, et al. Transcranial Doppler (TCD) ultrasonography in patients with ventriculomegaly: Investigation of additional parameters for qualifying shunt implantation. Polish Journal of Radiology. 2005 Jan 1;70(1):27–34.
• Lee G, Marszalek PE. UV radiation affects the mechanics of the double helix. BIOPHYSICAL JOURNAL. 2005 Jan 1;88(1):348A-348A.
• Zhang QM, Lee GR, Marszalek PE. Atomic cranks and levers control sugar ring conformations. Journal of Physics-Condensed Matter. 2005;17(18):S1427–42.
• Lee G, Nowak W, Jaroniec J, Zhang Q, Marszalek PE. Molecular dynamics simulations of forced conformational transitions in 1,6-linked polysaccharides. Biophysical journal. 2004 Sep;87(3):1456–65.
• Zhang QM, Jaroniec J, Lee G, Marszalek PE. Amylose elasticity depends on the solvent dielectric constant. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2004 Aug 22;228:U487–U487.
• Lu Z, Nowak W, Lee G, Marszalek PE, Yang W. Elastic properties of single amylose chains in water: a quantum mechanical and AFM study. Journal of the American Chemical Society. 2004 Jul;126(29):9033–41.
• Lee G, Nowak W, Jaroniec J, Zhang Q, Marszalek PE. Nanomechanical control of glucopyranose rotamers. Journal of the American Chemical Society. 2004 May;126(20):6218–9.
• Kersey FR, Lee G, Marszalek P, Craig SL. Surface-to-surface bridges formed by reversibly assembled polymers. Journal of the American Chemical Society. 2004 Mar;126(10):3038–9.
• Lee G, Nowak W, Marszalek PE. Mechanics of 1,6 linked polysaccharides by AFM and SMD calculations. BIOPHYSICAL JOURNAL. 2004 Jan 1;86(1):322A-322A.
• Lee G, Nowak W, Jaroniec J, Zhang QM, Marszalek P. Force-induced conformational transitions in 1,6-linked polysaccharides. Abstracts of Papers of the American Chemical Society. 2004;228:U225–U225.
• Marszalek PE, Oberhauser AF, Li H, Fernandez JM. The force-driven conformations of heparin studied with single molecule force microscopy. Biophysical journal. 2003 Oct;85(4):2696–704.
• Carrion-Vazquez M, Li H, Lu H, Marszalek PE, Oberhauser AF, Fernandez JM. The mechanical stability of ubiquitin is linkage dependent. Nature structural biology. 2003 Sep;10(9):738–43.
• Vazquez MC, Li HB, Lu H, Marszalek PE, Oberhauser AF, Fernandez JM. Linkage-dependent stability in polyubiquitin chains. BIOPHYSICAL JOURNAL. 2003 Feb 1;84(2):491A-492A.
• Nowak W, Lee G, Marszalek P. Mechanics of the glucopyranose ring by single molecule atomic force microscopy, quantum chemistry and molecular dynamics simulations. Abstracts of Papers of the American Chemical Society. 2003;226:U214–U214.
• Li HB, Carrion Vazquez M, Oberhauser AF, Marszalek P, Fernandez JM. Stretching single molecules into novel conformations using the atomic force microscope. Abstracts of Papers of the American Chemical Society. 2003;225:U685–U685.
• Li H, Linke WA, Oberhauser AF, Carrion-Vazquez M, Kerkvliet JG, Lu H, et al. Reverse engineering of the giant muscle protein titin. Nature. 2002 Aug;418(6901):998–1002.
• Marszalek PE, Li H, Oberhauser AF, Fernandez JM. Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM. Proceedings of the National Academy of Sciences of the United States of America. 2002 Apr;99(7):4278–83.
• Fernandez JM, Carrion Vasquez M, Li H, Oberhauser A, Marszalek P. Stretching single molecules into novel conformations using the atomic force microscope. Abstracts of Papers of the American Chemical Society. 2002;223:C30–1.
• Marszalek P. Fingerprinting polysaccharides with single-molecule atomic force microscopy (AFM). Abstracts of Papers of the American Chemical Society. 2002;224:U398–U398.
• Li HB, Linke WA, Oberhauser AF, Carrion-Vazquez M, Marszalek PE, Fernandez JM. Reconstitution of the passive elasticity of muscle from single molecule AFM experiments. BIOPHYSICAL JOURNAL. 2002;82(1):193A-193A.
• Marszalek P. Mechanical control of the pyranose ring conformations by single molecule AFM. Abstracts of Papers of the American Chemical Society. 2002;223:C37–C37.
• Marszalek PE, Li HB, Oberhauser AF, Fernandez JM. Chair-boat transitions in single polysaccharide molecules observed with force-clamp AFM. BIOPHYSICAL JOURNAL. 2002;82(1):41A-41A.
• Marszalek PE, Li H, Fernandez JM. Fingerprinting polysaccharides with single-molecule atomic force microscopy. Nature biotechnology. 2001 Mar;19(3):258–62.
• Marszalek PE, Oberhauser AF, Fernandez JM. Two-state model of polysaccharide elasticity probed with the force clamp mode of AFM. BIOPHYSICAL JOURNAL. 2001;80(1):154A-154A.
• Marszalek PE, Li HB, Fernandez JM. Fingerprinting polysaccharides with single molecule atomic force microscopy. BIOPHYSICAL JOURNAL. 2001;80(1):156A-156A.
• Li HB, Carrion-Vazquez M, Oberhauser AF, Marszalek PE, Fernandez JM. Point mutations alter the mechanical stability of immunoglobulin modules. BIOPHYSICAL JOURNAL. 2001;80(1):201A-201A.
• Li H, Carrion-Vazquez M, Oberhauser AF, Marszalek PE, Fernandez JM. Point mutations alter the mechanical stability of immunoglobulin modules. Nature structural biology. 2000 Dec;7(12):1117–20.
• Fisher TE, Carrion-Vazquez M, Oberhauser AF, Li H, Marszalek PE, Fernandez JM. Single molecular force spectroscopy of modular proteins in the nervous system. Neuron. 2000 Sep;27(3):435–46.
• Fisher TE, Marszalek PE, Fernandez JM. Stretching single molecules into novel conformations using the atomic force microscope. Nature structural biology. 2000 Sep;7(9):719–24.
• Marszalek PE, Greenleaf WJ, Li H, Oberhauser AF, Fernandez JM. Atomic force microscopy captures quantized plastic deformation in gold nanowires. Proceedings of the National Academy of Sciences of the United States of America. 2000 Jun;97(12):6282–6.
• Carrion-Vazquez M, Oberhauser AF, Fisher TE, Marszalek PE, Li H, Fernandez JM. Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering. Progress in biophysics and molecular biology. 2000 Jan;74(1–2):63–91.
• Li H, Oberhauser A, Fowler S, Clarke J, Marszalek PE, Fernandez JM. Mechanical unfolding of titin is under kinetic control. BIOPHYSICAL JOURNAL. 2000;78(1):402A-402A.
• Marszalek PE, Lu H, Li H, Carrion-Vazquez M, Oberhauser AF, Schulten K, et al. Mechanical unfolding intermediates in titin modules captured by single molecule AFM. BIOPHYSICAL JOURNAL. 2000;78(1):448A-448A.
• Oberhauser AF, Ohashi T, Marszalek PE, Erickson HP, Fernandez JM. The micromechanics of single fibronectin proteins studied with atomic force microscopy. BIOPHYSICAL JOURNAL. 2000 Jan 1;78(1):447A-447A.
• Czernicki Z, Dowzenko A, Andrychowski J, Głowacki M, Marszałek P. Combined treatment of cerebral aneurysms with utilization of clipping and coiling techniques. Neurologia i neurochirurgia polska. 2000 Jan;34(6 Suppl):21–6.
• Marszalek PE, Greenleaf WJ, Fisher TE, Oberhauser AF, Carrion-Vazuez M, Li H, et al. Mechanical properties of biological and inorganic mesoscopic systems studied with single molecule AFM. BIOPHYSICAL JOURNAL. 2000;78(1):10A-10A.
• Fisher TE, Oberhauser AF, CarrionVazquez M, Marszalek PE, Fernandez JM. The study of protein mechanics with the atomic force microscope (vol 24, pg 379, 1999). TRENDS IN BIOCHEMICAL SCIENCES. 2000;25(1):6–6.
• Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Force spectroscopy of cell adhesion molecules. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;2:1299.
• Marszalek PE, Oberhauser AF, Carrion-Vazquez M, Fernandez JM. Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;1:78.
• Marszalek PE, Lu H, Li H, Carrion-Vazquez M, Oberhauser AF, Schulten K, et al. Mechanical unfolding intermediates in titin modules. Nature. 1999 Nov;402(6757):100–3.
• Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Single protein misfolding events captured by atomic force microscopy. Nature structural biology. 1999 Nov;6(11):1025–8.
• Fisher TE, Marszalek PE, Oberhauser AF, Carrion-Vazquez M, Fernandez JM. The micro-mechanics of single molecules studied with atomic force microscopy. The Journal of physiology. 1999 Oct;520 Pt 1:5–14.
• Fisher TE, Oberhauser AF, Carrion-Vazquez M, Marszalek PE, Fernandez JM. The study of protein mechanics with the atomic force microscope. Trends in biochemical sciences. 1999 Oct;24(10):379–84.
• Carrion-Vazquez M, Marszalek PE, Oberhauser AF, Fernandez JM. Atomic force microscopy captures length phenotypes in single proteins. Proceedings of the National Academy of Sciences of the United States of America. 1999 Sep;96(20):11288–92.
• Marszalek PE, Pang YP, Li H, El Yazal J, Oberhauser AF, Fernandez JM. Atomic levers control pyranose ring conformations. Proceedings of the National Academy of Sciences of the United States of America. 1999 Jul;96(14):7894–8.
• Carrion-Vazquez M, Oberhauser AF, Fowler SB, Marszalek PE, Broedel SE, Clarke J, et al. Mechanical and chemical unfolding of a single protein: a comparison. Proceedings of the National Academy of Sciences of the United States of America. 1999 Mar;96(7):3694–9.
• Carrion-Vazquez M, Greenleaf WJ, Oberhauser AF, Marszalek PE, Fernandez JM. AFM measurements of protein unfolding: A molecular caliper with amino acid resolution. BIOPHYSICAL JOURNAL. 1999;76(1):A173–A173.
• Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Protein misfolding events captured by single molecule AFM spectroscopy. BIOPHYSICAL JOURNAL. 1999;76(1):A106–A106.
• Matyja E, Taraszewska A, Marszałek P. Necrosis and apoptosis of tumor cells in embolized meningiomas: histopathology and P53, BCL-2, CD-68 immunohistochemistry. Folia neuropathologica. 1999 Jan;37(2):93–8.
• Carrion-Vazquez M, Oberhauser AF, Fowler SB, Marszalek PE, Broedel SE, Clarke J, et al. AFM and chemical unfolding of a single protein follow the same pathway. BIOPHYSICAL JOURNAL. 1999;76(1):A173–A173.
• Marszalek PE, Oberhauser AF, Pang YP, Fernandez JM. The glucopyranose ring: An elastic switch disabled by periodate oxidation. BIOPHYSICAL JOURNAL. 1999;76(1):A352–A352.
• Marszalek PE, Oberhauser AF, Pang YP, Fernandez JM. Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. Nature. 1998 Dec;396(6712):661–4.
• Oberhauser AF, Marszalek PE, Erickson HP, Fernandez JM. The molecular elasticity of the extracellular matrix protein tenascin. Nature. 1998 May 14;393(6681):181–5.
• Marszalek PE, Oberhauser AF, Pang YP, Fernandez JM. The glucopyranose ring: An elastic switch disabled by periodate oxidation. MOLECULAR BIOLOGY OF THE CELL. 1998;9:198A-198A.
• Marszalek PE, Farrell B, Verdugo P, Fernandez JM. Kinetics of release of serotonin from isolated secretory granules. I. Amperometric detection of serotonin from electroporated granules. Biophysical journal. 1997 Sep;73(3):1160–8.
• Marszalek PE, Farrell B, Verdugo P, Fernandez JM. Kinetics of release of serotonin from isolated secretory granules. II. Ion exchange determines the diffusivity of serotonin. Biophysical journal. 1997 Sep;73(3):1169–83.
• Xie TD, Chen Y, Marszalek P, Tsong TY. Fluctuation-driven directional flow in biochemical cycle: further study of electric activation of Na,K pumps. Biophysical journal. 1997 Jun;72(6):2496–502.
• Marszałek P, Jurkiewicz J, Fersten E, Luczywek E, Czernicki Z, Gielecki J, et al. [Multi-stage method for the diagnosis of low-pressure hydrocephalus]. Neurologia i neurochirurgia polska. 1997 May;31(3):523–35.
• Farrell B, Marszalek P, Fernandez JM. Scaling laws of amine release: Role of the exocytotic fusion pore. Biophysical Journal. 1997;72(2):TUP98–TUP98.
• Marszalek P, Farrell B, Fernandez JM. Ion-exchange gel regulates neurotransmitter release through the exocytotic fusion pore. Society of General Physiologists series. 1996 Jan;51:211–22.
• Marszalek P, Farrell B, Fernandez J. Kinetics of the release of serotonin from isolated secretory granules. Biophysical Journal. 1996;70(2):MPME5–MPME5.
• Marszalek PE, Markin VS, Tanaka T, Kawaguchi H, Fernandez JM. Pulsed Laser Imaging Demonstrates the Mechanism of Current Rectification at a Hydrogel Interface. Langmuir. 1995 Nov 1;11(11):4196–8.
• Marszalek PE, Markin VS, Tanaka T, Kawaguchi H, Fernandez JM. The secretory granule matrix-electrolyte interface: a homologue of the p-n rectifying junction. Biophysical journal. 1995 Oct;69(4):1218–29.
• Marszalek P, Tsong TY. Cell fission and formation of mini cell bodies by high frequency alternating electric field. Biophysical journal. 1995 Apr;68(4):1218–21.
• Xie TD, Tsong TY, Chen YD, Marszalek P. Properties of Electroconformational Coupling (Ecc) - Activation of Na,K-Atpase by Electric Pulses of Fluctuating Lifetime and Fluctuating Amplitude. Faseb Journal. 1995;9(6):A1339–A1339.
• Xie TD, Marszalek P, Chen YD, Tsong TY. Recognition and processing of randomly fluctuating electric signals by Na,K-ATPase. Biophysical journal. 1994 Sep;67(3):1247–51.
• Tsong TY, Xie TD, Marszalek P, Chen YD. Absorption of Free-Energy from Fluctuating Electric-Fields by a Membrane Ion-Pump to Perform Chemical Work. Biophysical Journal. 1994;66(2):A236–A236.
• Pawłowski P, Szutowicz I, Marszałek P, Fikus M. Bioelectrorheological model of the cell. 5. Electrodestruction of cellular membrane in alternating electric field. Biophysical journal. 1993 Jul;65(1):541–9.
• Gimsa J, Marszalek P, Loewe U, Tsong TY. Electroporation in rotating electric fields. Bioelectrochemistry and Bioenergetics. 1992 Jan 1;29(1):81–9.
• Gimsa J, Marszalek P, Loewe U, Tsong TY. Dielectrophoresis and electrorotation of neurospora slime and murine myeloma cells. Biophysical journal. 1991 Oct;60(4):749–60.
• Marszalek P, Zielinsky JJ, Fikus M, Tsong TY. Determination of electric parameters of cell membranes by a dielectrophoresis method. Biophysical journal. 1991 May;59(5):982–7.
• Marszalek P, Liu DS, Tsong TY. Schwan equation and transmembrane potential induced by alternating electric field. Biophysical journal. 1990 Oct;58(4):1053–8.
• Zieliński JJ, Marszałek P, Fikus M. A new method for the investigation of cellular dielectrophoresis. Zeitschrift fur Naturforschung C, Journal of biosciences. 1989 Sep;44(9–10):845–8.
• Marszalek P, Zieliński JJ, Fikus M. Experimental verification of a theoretical treatment of the mechanism of dielectrophoresis. Journal of Electroanalytical Chemistry. 1989 Jan 1;276(3):289–98.
• Marszałek P, Zieliński JJ, Fikus M. Experimental verification of a theoretical treatment of the mechanism of dielectrophoresis. Bioelectrochemistry and Bioenergetics. 1989 Jan 1;22(3):289–98.
• Fikus M, Marszalek P, Rozycki S, Zielinski J. Dielectrophoresis and Electrofusion of Neurospora-Crassa Slime. Studia Biophysica. 1987;119(1–3):73–6.
• Marszałek P, Godzik A. The Monte Carlo simulation of pearl chain formation. Radiation and environmental biophysics. 1987 Jan;26(4):313–7.
• Fikus M, Grzesiuk E, Marszalek P. Electrofusion of Neurospora crassa slime cells. FEMS Microbiology Letters. 1985 Jan 1;27(1):123–7.
• Fikus M, Grzesiuk E, Marszałek P, Rózycki S, Zieliński J. Electrofusion of Neurospora crassa slime cells. FEMS Microbiology Letters. 1985 Jan 1;27(1):123–7.